Protein Array Fabrication with E-jet Printing

We demonstrate the feasibility of fabricate biosensors with direct protein printing without complicated processes at high resolution and area coverage. Printed single protein, streptavidin-fluorescein, binds to biotin labeled with fluorescent DNA without denaturation; therefore a fluorescence microscope can detect the printed droplets as shown in Figure 7.1(a). The standard deviation of the spot size and spacing is 7.26 % and 0.693%, respectively. The e-jet printing is capable to print large area, e.g., one inch size. Half droplets of streptavidin-Cy5 are printed in 15 minutes (Figure 7.1(b)). Four-inch size printing is also available. Moreover, we have developed the multi-syringe head [1]. The head prints multiple inks by rotation. Even complicated patterns of multiple proteins (e.g., peacock shown in Figure 7.1(c)) can be printed. Printed primary antibodies can be detected under a fluorescence microscopy by incubating with fluorescently-labeled secondary antibodies [2]. These capabilities show that the e-jet printer with the multi-syringe head has feasibility to make protein chips to detect multiple proteins at lower cost than the conventional chips

Alien Registration- Rogers, John A. (Rumford, Oxford County)

https://digitalmaine.com/alien_docs/13552/thumbnail.jp

Cyanide practice

The ENCINO CYANIDE PLANT was erected for the treatment of old accumulated tailings, which were worked by the Patio and Cazo processes. As with most accumulated tailings, which have been exposed to the action of the air for a considerable period, they have undergone changes, whereby, forming compounds which are highly detrimental to a solution of Potassium Cyanide and the direct cause of the present high consumption of Cyanide --page 1

Standardized Assessments, Care Planning, and Improved Quality of Life for Residents of Adult Family Homes

Quality of life for adults living in adult family homes requires further attention as the elderly population continues to grow and moves from minority to majority. There is a lack of data on residents in adult family home settings and their quality of life. The purpose of this project was to identify if individualized nursing care plans had an impact on the residents\u27 quality of life in the adult family home. In this study, a registered nurse assessed the residents to create an individualized nursing care plan that would be implemented to improve quality of life. These care plans were comprised of 3 distinct nursing needs: risk of falls, self-care deficit, and nutrition imbalance less than the body needs. Each care plan was created with nursing-specific interventions that could be tested, replicated, and evaluated. A test of this premise was conducted using a sample of 6 residents in an adult family home. Only one resident met the criteria of mild to moderate dementia for testing. The single qualifying resident who participated in the project demonstrated improvement in her quality of life after the proposed intervention was implemented. This change was evaluated using quantitative data gathered with the Dementia Quality of Life assessment, and qualitatively with the caregiver and registered nurse evaluation tool. The interventions of the nursing care plans were relevant and as a result, the Dementia Quality of Life assessment did show an improvement in scores, which reflected an improvement in quality of life. Elderly adults with dementia living in an adult family home may benefit from individualized nursing care plans, which may improve their quality of life

Restructuring's Effect on Related and Unrelated Diversification Among Top Food Manufacturing Firms in the 1980s

Corporate restructuring during the 1980s is argued to have focused on improving firm performance by increasing related and decreasing unrelated diversification. The restructuring patterns of top food manufacturing firms do not support this; instead, much of the restructuring appears to have been driven by the pursuit of stronger market positions. TheAgribusiness, Industrial Organization,

Applications of artificial intelligence to mission planning

The scheduling problem facing NASA-Marshall mission planning is extremely difficult for several reasons. The most critical factor is the computational complexity involved in developing a schedule. The size of the search space is large along some dimensions and infinite along others. It is because of this and other difficulties that many of the conventional operation research techniques are not feasible or inadequate to solve the problems by themselves. Therefore, the purpose is to examine various artificial intelligence (AI) techniques to assist conventional techniques or to replace them. The specific tasks performed were as follows: (1) to identify mission planning applications for object oriented and rule based programming; (2) to investigate interfacing AI dedicated hardware (Lisp machines) to VAX hardware; (3) to demonstrate how Lisp may be called from within FORTRAN programs; (4) to investigate and report on programming techniques used in some commercial AI shells, such as Knowledge Engineering Environment (KEE); and (5) to study and report on algorithmic methods to reduce complexity as related to AI techniques

A Biblical-Theological Framework for Human Sexuality: Applications to Private Sexuality

What are good sexual acts? It is not that surprising when cultural voices, without reference to God, argue for the inherent goodness of all “unharmful” sexual desires and acts. Regrettably, ethical pragmatism has influenced some Christian sexual ethics, and this influence is particularly evident with the issue of masturbation. What God defines as good sexual acts are those that fulfill his unitive and procreative purposes for sex within marriage. Given God’s unitive and procreative purposes for sex within the context of marriage, we argue that masturbation is a categorically impermissible act because it fulfills neither of these purposes, and we counter Christian arguments for its permissibility. God calls Christians to deal with sexual desires, including good sexual desires, through either marital sexual expression or Spirit-enabled self-control

Protein synthesis in skeletal muscle following acute exhaustive exercise

Cell-free and whole-tissue protein synthesis was studied in skeletal muscle of untrained male guinea pigs that had undergone a treadmill run to exhaustion. Experiments using explants from the gastrocnemius muscle maintained in organ culture demonstrated that the ability of the acutely exercised muscle to incorporate amino acids into protein had increased. Compared to polyribosomes prepared from several lower hind limb muscles of nonexercised guinea pigs, polyribosomes from the same muscle in exhausted guinea pigs had incorporated almost 50% more radioactive leucine into protein. However, the polysome profiles of control and exercised muscle were identical, and no difference in the total polysome RNA content could be detected. The efficiency of in-vitro protein synthesis using washed membrane-bound polyribosomes (microsomes) isolated from acutely exercised skeletal muscle was 50% greater than with microsomes from rested control muscle.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/50128/1/880020403_ftp.pd

Contact plane concentration

Contact plane concentration depends upon the principle that a thin sheet or film of water flowing over a plane surface may be so adjusted that its quantity and velocity are just sufficient to bear away the lighter particles of gangue, leaving behind only the heavier particles of the mineral to be concentrated, adhering by friction to the plane, the concentrates being removed by any convenient method. Thus we see it is best adapted to the concentration of minerals having widely differing specific gravities but may be used for the concentration of all ores and minerals with any specific gravity whatsoever --page 1